Notch deficiency mediates arterial valve defects in bmp16 mutants. (A) WISH analysis of notch1b expression in wild-type and bmp16 knockout embryos at 72 hpf. Scale bar: 100 μm. (B) qRT-PCR analysis of notch1b in adult hearts of wild-type and bmp16 knockout zebrafish. The statistical analysis was performed using unpaired Student’s t-test. (C) WISH analysis of notch1b expression in vehicle- or LDN-193189-treated embryos at 72 hpf and 96 hpf. Scale bar: 100 μm. (D) qRT-PCR analysis of notch1b in dissected embryonic hearts at 96 hpf following vehicle or LDN-193189 treatment. The statistical analysis was performed using unpaired Student’s t-test. (E) Schematic representation of the timeline for Yhhu-3792 treatment. (F) Time-lapse LSFM images of Tg(gata1:DsRed) embryos in wild-type or bmp16 knockout background treated with DMSO or Yhhu-3792 at 96 hpf. Individual blood cells were pseudocolor-coded for tracking. Scale bar: 20 µm. (G) Quantification of blood cells regurgitating from the bulbus arteriosus into the ventricle in wild-type and bmp16 knockout embryos treated with DMSO or Yhhu-3792. The statistical analysis was performed using two-way ANOVA. n = 7–11 per group. *, p < 0.05; **, p < 0.01; ****, p < 0.0001.
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