FIGURE

Fig. 2.

ID

ZDB-FIG-260311-151

Publication

Kamei et al., 2026 - Reciprocal inhibition of Wnt signaling pathways pattern the interconnection of epithelial tubules in the regenerating zebrafish kidney

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Fig. 2.

Canonical Wnt signaling highlights the point of tubule invasion. (A,B) Canonical Wnt target genes lef1 (A) and wnt4 (B) are expressed in new nephron tubules (marked by EdU incorporation) at the tubule fusion junction with the distal tubule (DT; dotted outline) marked by Tg(lhx1a:eGFP) expression. (C) The Tg(tcflef:dGFP) Wnt reporter transgene is expressed at the point of tubule fusion. (D,E) The canonical Wnt inhibitor IWR1 blocks formation of invasive basal protrusions (arrowheads). (F) Quantification of basal protrusion phenotype by Kappa curvature analysis in ImageJ reveals loss of protrusions after IWR1 treatment (Mary and Brouhard, 2019 preprint; see Materials and Methods). Green in B is autofluorescence. Blue fluorescence is Hoechst-stained nuclei. Scale bars: 5 µm. **P=0.0012 (Mann-Whitney test). Data are mean±s.d. (control: 0.6986±0.2750, n=9; IWR1 median: 0.2204±0.1136, n=7).

Expression Data

Genes:	EGFP lef1 wnt4
Fish:	kyu1Tg/kyu1Tg pt303Tg/pt303Tg
Condition:	chemical ablation: gentamycin: kidney
Anatomical Term:	regeneration epithelium renal tubule
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	pt303Tg/pt303Tg
Conditions:	chemical ablation: gentamycin: kidney chemical ablation: gentamycin: kidney, chemical treatment by environment: IWR-1-endo
Observed In:	kidney epithelium regeneration nephron progenitor tube formation regeneration epithelium renal tubule
Stage:	Adult

Phenotype Detail

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