FIGURE

Figure 3

ID

ZDB-FIG-260215-136

Publication

Ogmen et al., 2025 - Insights into KIF11 pathogenesis in Microcephaly-Lymphedema-Chorioretinopathy syndrome from a lymphatic perspective

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Figure 3

KIF11 expression during human embryonic development and transcriptional control in adult lymphatic endothelial cells.

(A) Human embryonic slides corresponding with different embryonic developmental Carnegie stages (CS12, CS15, CS18, and CS22) were hybridized with fluorescence probes for KIF11 (white), PROX1 or PDPN (green), and FLT4 (red). Magnified regions marked with a square box in overview images contain the precardinal veins (PCVs), cardinal veins (CVs), and jugular lymphatic sacs (JLSs), and arrows point to regions of coexpression between KIF11 and lymphatic endothelial marker expression. HP, hepatic primordia. Scale bar: 200 μm. (B) ChIP-Seq analysis in cultured human dermal LECs identified an intronic region of KIF11 bound by FOXC2 and NFATC1 (top panel) and PROX1 binding 1.3 kb upstream of KIF11 promoter (bottom panel). (C) LECs were treated with siRNA PROX1 or siRNA FOXC2, and KIF11 expression was evaluated by qPCR. Bars represent mean relative expression ± SEM from n = 3 experiments. **P < 0.01, ***P < 0.001, ****P < 0.0001. Two-tailed unpaired Student’s t test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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