Description and development of adult zebrafish anterior lateral line innervation. (A–A′) Innervation map at early larval stages (3 mm SL/7 dpf). (A) 3 mm SL nerve map schematic, based on adjacent image of anterior lateral line afferent innervation of a 3 mm SL specimen immunolabeled for anti‐GFP against Tg(Hgn39d:GFP) (confocal maximum projection). The schematic shows the anterodorsal nerves (nAD) and ganglia in cyan (nAD supraorbital [so] and infraorbital [io]) and the anteroventral nerves (nAV) in magenta (nAV mandibular [md] and opercular [op]). Axonal projections to the hindbrain are indicated in purple. (A′) Close up of late forming superficial nerves (red labels) in the infraorbital region, running parallel to canal nerves, and neuromasts (black labels) at 3 mm SL. (B) 15 mm SL nerve map schematic showing the mature anterodorsal system (cyan: nAD supraorbital, infraorbital, and anterior pit [ap]) and anteroventral system (magenta: nAV mandibular, mandibular superficial [mds], infraorbital superficial [ios], opercular, opercular dorsal [opd], and supraorbital superficial [sos]), based on adjacent image of innervation of a 15 mm SL specimen immunolabeled for anti‐GFP against Tg(Hgn39d:GFP) (lightsheet maximum projection). (C) 10 mm SL specimen, showing nerve branch points from the anteroventral ganglion (confocal maximum projection at high magnification). The nAVsos branch point is indicated with a red arrow. Scale = 50 µm. (D–E) Innervation map at adult stages (22 mm SL) immunolabeled for anti‐GFP against Tg(Hgn39d:GFP). (D) Lightsheet maximum projection of orbital region of the cranium showing the anterodorsal ganglion and nerves (cyan in adjacent schematic). (E) Lightsheet maximum projection of lateral‐ventral view of a 22 mm SL specimen showing the anteroventral nerves (magenta in adjacent schematic). These adult specimens were cleared with CUBIC and immunolabeled with anti‐GFP antibody to enhance the endogenous Tg(Hgn39d:GFP) signal. Scale = 100 µm.
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