Figure 4

Stc2a regulates somatic growth in an IGF signaling-dependent manner. (A) Whole body phospho-Akt levels. Lysates of pooled wild-type (WT) and stc2a(Δ2 + 4)^-/- larvae (3 dpf) were analyzed by Western blotting using antibodies against phospho-Akt, total Akt, and GAPDH. Representative images are shown in the left panel; quantified results are shown in the middle and right panels. n = 7, ns, no significant, t -test. (B) The same samples described in (A) were analyzed using phospho-ERK, total ERK levels, and GAPDH. n = 7, ns, no significant, t-test. (C) Fish of the indicated genotypes were treated with 1.5 μM BMS-754807 or vehicle from 6–51 hpf. Body length was measured individually. Percent decrease caused by BMS treatment was calculated and shown. n = 58~68. ****, P < 0.001, t-test. (D) Fish of the indicated genotypes were treated with 8 μM ZnCl₂ from 6–51 hpf. Body length was measured individually. Percent decrease caused by ZnCl₂ was calculated and shown. n = 36~43. **, P <0.01, t-test. (E, F) Fish of the indicated genotypes were treated with 0.3 μM wortmannin (E) or 8 μM U0126 or vehicle from 6–51 hpf. Body length was measured individually. Percent decrease caused by each drug was calculated and shown. n = 37-72. ****p < 0.0001, t-test. In all above panels, data shown are mean ± SEM.