Loss of Stc2a increases developmental speed and growth rate, resulting in enlarged adult organ and body size. (A) Gross morphology of fish of the indicated genotypes at the indicated stages. Lateral views with anterior to the left and dorsal up. Scale bar = 0.2mm. dpf, day post fertilization. (B, C). Body length (B) and head-trunk angle (HTA) (C) of the indicated mutant fish and wild-type (WT) zebrafish at 51 hpf. WT, n = 76, stc2a(Δ2 + 4)-/-, n = 73, and stc2a(Δ5)-/-, n = 10. ****p < 0.0001 by one-way ANOVA followed by Tukey’s multiple comparisons test. (D) The somite number of the indicated fish line at 26 hpf. WT, n = 40, stc2a(Δ2 + 4)-/-, n = 25, and stc2a(Δ5)-/-, n = 14. ****p < 0.0001 by one-way ANOVA followed by Tukey’s multiple comparisons test. (E, F) Body length (E) and body weight (F) of the indicated genotype of 6 month-old. WT, n = 16, stc2a(Δ2 + 4)+/-, n = 14, and stc2a(Δ5)-/-, n = 15. **p < 0.01, ****p < 0.0001 by one-way ANOVA followed by Tukey’s multiple comparisons test. (G–H) Dorsal spine length (G) and pelvic spine length (H) of the indicated genotypes of 1 year-old. Fish were stained by alizarin red and a representative image is shown on the left and quantitative results on the right. *p < 0.05 by unpaired two-tailed t-test. stc2a(Δ2 + 4)+/- n = 6, and stc2a(Δ5)-/-, n = 8. In all above panels, data shown are mean ± SEM.
|
