Fig 6

Dancr is essential for zebrafish embryogenesis.

(A, B)Dancr del F0 crispant embryos display multiple overt developmental defects. The dancr promoter was deleted in zebrafish using CRISPR-Cas9 genome editing and F0 crispant embryos were analysed at 24 hpf. (A) Phenotypic abnormalities were separated into four classes based on their severity and representative images of the different classes are shown. Embryos are presented laterally. Scale bar = 200μm. (B) The number of abnormal embryos was quantified for dancr F0 crispant, uninjected wild type sibling and mock control embryos. Results are presented as a percentage of total embryos. (C) Zebrafish dancr transcript partially rescues the effect of dancr promoter deletion whilst (D)snora26, snora27 and human DANCR co-injection increase the severity of the F0 dancr crispant phenotype. The indicated amounts of in vitro transcribed lncRNA or snoRNA were co-injected with the Cas9-crRNA-tracrRNA RNP complex in a genome editing experiment. The total number of F0 dancr crispant embryos in each class was quantified at 24 hpf and presented as a percentage of total embryos. Two-tailed two sample t-test p < 0.05*, p < 0.01**, p < 0.001***, p < 0.0001****.