Fig 2

DANCR is a clinically important MITF and c-MYC regulated lncRNA in human melanoma.

(A) UCSC genome browser view showing MITF and c-MYC ChIP-seq peaks across the DANCR locus (GRCh37/hg19). (B) MITF and (C) c-MYC regulate DANCR in melanoma. siRNA transfection was used to deplete MITF and c-MYC levels in 501mel, A375 and SK-MEL-28 cells. Expression changes were analysed using RT-qPCR. POLII was used as a reference gene. Results presented as mean + /- SEM., n ≥ 2; Two-tailed two sample t-test p < 0.05*, p < 0.01**, p < 0.001***. Individual dots represent separate biological replicates. (D)DANCR is highly expressed in all genomic subtypes of melanoma. Box and whisker plot (min to max; showing all points) representing DANCR expression in NRAS, NF1, BRAF, and triple negative genomic subtypes of melanoma using TCGA Skin Cutaneous Melanoma (SKCM) RNA-seq datasets. (E) Melanoma patients with high DANCR expression have significantly decreased survival. TCGA SKCM patients were sorted based on DANCR levels. Percent survival was compared between DANCR high (top third) and DANCR low (bottom third) groups. Cox regression analysis shows that high DANCR expression correlates with statistically significant decreased survival (logrank p-value = 0.00628).