Fig 5

elf3^−/− adults present immune-related pathologies and exhibit poor survival. (A, B) Venn diagram of observed clinical signs of health deterioration, such as abnormal swimming, erythema, and ulcer development in elf3^+/+ (A) and elf3^−/− (B) adults. (C) Comparison of the genotypic ratios observed in adult zebrafish >90 dpf generated from heterozygous incrosses of elf3^+/− parents to expected Mendelian outcomes. (D) Representative images of severe (bottom) and milder (middle) ulcers in elf3^−/− adults compared to healthy elf3^+/+ adults (top). (E) Kaplan-Meier survival curves of co-housed elf3 genotypes starting from the initial observation at 60 dpf (dotted line) and ending at 371 dpf. Repeated genotyping events occurred at 60 dpf, 100 dpf, 277 dpf, and 368 dpf. (F) Kaplan-Meier survival curves of separately housed elf3^+/+ and elf3^−/− adults starting from the initial observation at 60 dpf (dotted line) and ending at 371 dpf. P-values were calculated for panel A using a chi-square goodness-of-fit test and for panels E and F using an overall log-rank test followed by Bonferroni-corrected log-rank tests for individual comparisons when required.