Human AP1G1 MUT mRNA expression induces neuronal defects during early embryonic development. (A) Expression of ngn1 at 48 hpf was analyzed by whole-mount in situ hybridization (WISH) in embryos injected with either human WT or MUT AP1G1 mRNA. Representative images from three independent experiments, with 20 embryos per condition in each replicate (total number of embryos with the described phenotype is shown in red for each experimental condition). Dorsal views are shown at 20× magnification. Abbreviations: fb, forebrain; hb, hindbrain; mb, midbrain. (B) The graphic shows measurements for the forebrain, midbrain and hindbrain area analyzed using ImageJ software (Version 1.8.0). (C) The drawing represents the areas (colored differently) that were identified for the measurements. Three distinct experiments were performed, with 10 embryos per treatment (30 embryos for each experimental condition) considered for analysis. Data are presented as violin plots. Inside each violin, the central dashed line marks the median (Q2), and the superior and inferior dotted lines mark the third (Q3) and first (Q1) quartiles. Statistical significance among and between groups was calculated using one-way ANOVA with post hoc Tukey’s multiple comparison test (**** = p < 0.0001).
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