FIGURE

Figure 5

ID

ZDB-FIG-251115-139

Publication

Imperatore et al., 2025 - Usmani-Riazuddin Syndrome: Functional Characterization of a Novel c.196G>A Variant in the AP1G1 Gene and Phenotypic Insights Using Zebrafish as a Vertebrate Model

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Figure 5

Survival and malformation rates of embryos injected with human AP1G1 WT and MUT mRNA. The X-axis shows the survival rate (%) of embryos, while the Y-axis shows the mRNA concentration injected in a final volume of 4 nl at the following concentrations: 10, 50, 100, and 250 pg/embryo. CTRL (−) represents not-injected-embryos. At the same developmental stages, the morphology of embryos injected with human AP1G1 WT or MUT mRNA was carefully evaluated by visual microscopic inspection, considering changes in brain regions, tail morphology, the presence of pericardial edema, and notochord malformations. (A,B) Survival and malformations were analyzed at 48 and 144 hpf. (C) In Figure 5C are shown representative embryos at both developmental stages and injected with 100 and 250 pg/embryo of both human WT and MUT AP1G1 mRNA. The results represented in (A–C) panel are expressed as the mean ± SD of four independent experiments, with 30 embryos for each experiment and treatment. (*** p < 0.001 vs. control group; **** p < 0.0001 vs. control group; ns = not significant).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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