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Cardiac morphological changes in tomato fish. A Representative images of hearts extracted from wild-type (WT) and tomato zebrafish at 3 months post-fertilization (mpf). Scale bars, 500 μm. B Heart areas (ventricle + atrium) of WT and tomato zebrafish. N = 8 fish in each group. C The area of the ventricle in WT and tomato zebrafish. N = 8 zebrafish in each group. D–I Masson’s trichrome-stained sections. Horizontal (D, E) and coronal sections (F, G). Blue dotted lines: atrium. Scale bar, 500 μm. H, I Magnified images of AV valve in F, G, respectively. Dark blue dotted lines: atrioventricular (AV) valves. Scale bar, 50 μm. A atrium, V ventricle, arrow: AV valve. J Quantification of AV valve thickness. Three consecutive sections of each zebrafish were analyzed. The AV valve thickness was measured at its widest point in each section, and the average of these measurements was used as an indicator of valve thickness. Each dot represents a single zebrafish. N = 4 in each group. Data were presented as mean ± SEM. Statistics: unpaired two-tailed Student's t-test in (B, C, J).
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