FIGURE

Fig. 1

ID

ZDB-FIG-251021-55

Publication

Gonzalez et al., 2025 - Synergistic and independent roles for Nodal and FGF in zebrafish CPC migration and asymmetric heart morphogenesis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

FGF signaling is active in the developing heart and necessary for proper heart development. (A) RNA ISH by hybridization chain reaction for fgf8 (magenta) and myl7 (green) in the cardiac cone of a 20 hpf WT embryo. Scale bars: 25 µm. (B) Representative images of 48 hpf WT embryos or embryos treated with the FGF inhibitor SU5402 from 19 to 30 hpf. Magenta dashed lines outline the ventricle, cyan dashed lines the atrium. SU5402-treated embryos display aberrant cardiac looping, misshapen and misplaced chambers, and pericardial edema. (C) ISH for the Nodal target gene lefty2 (lft2) in the cardiac cone of 20 hpf embryos shows correct left-sided lefty2 and incorrect right, bilateral or absent lefty2 expression. (D) Quantification of lefty2 expression sidedness. WT, n=74; ntl−/−, n=50; spaw−/−, n=36; SU5402, n=41; dn-fgfr1, n=20; ntl−/−+SU5402, n=177; spaw−/−+SU5402, n=63. ***P<0.0001 (Chi-square analysis). ns, not significant. (A,C) Images are dorsal views, with anterior to the top and left to the reader's left.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development