FIGURE

Fig. 6

ID

ZDB-FIG-251006-53

Publication

Li et al., 2025 - EMB is essential for enteric nervous system development mediated by PI3K signaling

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Fig. 6

EMB activates PI3K pathway by recruiting PP2A to the cell membrane. A, Co-IP between EMB and PPP2CA in N-2a cells. B, Immunofluorescence staining of EMB (red) and PPP2CA (green) in WT or Emb-/- N-2a or 293 T cells. The yellow puncta indicate co-localization (C), Ppp2ca mRNA expression levels determined by RT-qPCR. D, Upon fractionation, western blots of total and phosphorylated membrane-associated Ppp2ca in 293 T or N-2a cells. E and F, Quantification of the protein levels in panel D. G and H, The Serine/threonine phosphatase activities of PP2A enzymes in the cytoplasmic fraction. ns, not significance; *, p < 0.05; ***, p < 0.001. I, A working model of EMB function in PI3K signaling

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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