FIGURE

Fig. 1

ID

ZDB-FIG-250917-57

Publication

Tevar et al., 2025 - Zebrafish adamtsl4 knockout recapitulates key features of human ADAMTSL4-related diseases: a gene involved in extracellular matrix organization, cell junctions and development

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

Molecular characterization of the adamtsl4-KO zebrafish line generated using CRISPR/Cas9 genome editing. A. Localization of the deletion identified in adamtsl4 exon 3. Scissors: DNA cleavage sites targeted by two crRNAs (crRNA1.1 and crRNA1.2). The numbers indicate the cDNA nucleotide positions corresponding to the start and end of the deleted DNA fragment. Wild-type sequences are highlighted with a green background, while deleted sequences are marked with a red background. Arrows: position of the forward and reverse primers used for zebrafish genotyping. Rectangles in the electropherograms mark the 5′ and 3′ ends of the deletion. B. Reduced mRNA levels in adamtsl4-KO zebrafish larvae. Relative adamtsl4 mRNA levels were measured in 6 days post-fertilization (dpf) zebrafish larvae using RT-qPCR. Data represent the mean ± standard error, derived from three independent experiments performed in triplicate, using pools of 50 larvae. Statistically significant differences between KO and wild-type zebrafish are denoted by asterisks: p < 0.05 (∗), p < 0.01 (∗∗). (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Expression Data

Gene:	adamtsl4
Fish:	AB adamtsl4^ucl6/ucl6 (AB) adamtsl4^ucl6/+ (AB)
Anatomical Term:	whole organism
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	adamtsl4^ucl6/ucl6 (AB) adamtsl4^ucl6/+ (AB)
Observed In:	whole organism
Stage:	Day 6

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Experimental Eye Research, , Tevar, A., Aroca-Aguilar, J.D., Atiénzar-Aroca, R., Ramírez, A.I., Fernández-Albarral, J.A., Escribano, J., Zebrafish adamtsl4 knockout recapitulates key features of human ADAMTSL4-related diseases: a gene involved in extracellular matrix organization, cell junctions and development, 110572110572, Copyright (2025) with permission from Elsevier. Full text @ Exp. Eye. Res.