FIGURE

Fig. 2

ID

ZDB-FIG-250902-46

Publication

Sugitani et al., 2025 - The Rapid Activation of MYDGF Is Critical for Cell Survival in the Acute Phase of Retinal Regeneration in Fish

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Fig. 2

MYDGF-producing cells in the zebrafish retina 1 h after ONI. (a) Double fluorescence staining of MYDGF (green) and nuclear staining with DAPI (blue). The MYDGF-positive cells included photoreceptors in the ONL, the border of the ONL and OPL, and the cells in the IPL (Figure 2a). (b) Fluorescent double staining of the photoreceptor marker proteins rhodopsin (red) and MYDGF (green). (c) Triple fluorescence staining of MYDGF (green), nuclear staining with DAPI (blue), and leukocyte marker Lcp1 (red). Lcp1 clearly colocalized with the MYDGF-positive distribution at the ONL-OPL border regions and in the IPL. (d) The microglial marker protein Iba1 colocalized with the MYDGF-positive region at the ONL-OPL border regions (indicated by the arrowhead). (e) GFAP-positive Müller cells showed almost no co-localization with MYDGF. (f) The pigment epithelium (PE), observed as black under brightfield microscopy, was found to lack MYDGF production. Representative images from 3–4 independent experiments of IHC are shown. Scale bar = 50 μm.

Expression Data

Gene:	mydgf
Fish:	WT
Condition:	compression injury: cranial nerve II
Anatomical Terms:	retinal inner plexiform layer retinal inner plexiform layer leukocyte retinal outer nuclear layer retinal outer nuclear layer leukocyte retinal outer nuclear layer photoreceptor cell retinal outer plexiform layer retinal outer plexiform layer leukocyte
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT
Condition:	compression injury: cranial nerve II
Observed In:	retinal inner plexiform layer retinal outer nuclear layer retinal outer plexiform layer
Stage:	Adult

Phenotype Detail

Acknowledgments

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