Figure 6—figure supplement 1.

(A) Histology is shown for two additional fish from each injection group. Dotted lines indicate the site of sectioning. Red chromogen was used for all immunohistochemical (IHC) staining. Scale bars, 50 µm. (B) Sox10 intensity per nucleus from NT and sox10 KO tumors. Intensity was calculated from immunofluorescence staining of Sox10. Red lines indicate mean with 95% CI. Each point represents one nucleus. NT-1 n=1334 cells, NT-2 n=1606 cells, NT-3 n=1204 cells, sox10-1 n=869 cells, sox10-2 n=998, sox10-3 n=1046. Cells from n=3 images were analyzed for each tumor. (C) UMAP of scRNA-seq dataset for MM074 cell line from Wouters et al. Cells from siSOX10 and siNT conditions are labeled. (D) Normalized expression of SOX10 per cell in UMAP space in MM074 cell line. (E) Normalized expression of SOX9 per cell in UMAP space in MM074 cell line. (F) Violin plots of normalized expression of SOX10 per cell. Median is shown as a dashed red line. Wilcoxon rank sum test was used to assess statistical significance, ****p<0.0001. (G) Violin plots of normalized expression of SOX9 per cell. Wilcoxon rank sum test was used to assess statistical significance; ns: no significance. (H) UMAP of scRNA-seq dataset for MM087 cell line from Wouters et al. Cells from siSOX10 and siNT conditions are labeled. (I) Normalized expression of SOX10 per cell in UMAP space in MM087 cell line. (J) Normalized expression of SOX9 per cell in UMAP space in MM087 cell line. (K) Violin plots of normalized expression of SOX10 per cell. Median is shown as a dashed red line. Wilcoxon rank sum test was used to assess statistical significance, ****p<0.0001. (L) Violin plots of normalized expression of SOX9 per cell. Wilcoxon rank sum test was used to assess statistical significance, ****p<0.0001.