Fig. 3

Depletion of macrophages (Μϕ) impairs intestinal regeneration. (A) Experimental design for the co-ablation of Μϕ and IECs in zebrafish larvae in the double reporter Tg(cldn15la:NTR-EGFP; mpeg1:NTR-mCherry). (B) Μϕ depletion efficiency (in percentage) of IEC + Μϕ co-ablated group after Mtz treatments. Scale bar = 500 µm. Pooled data of 3 independent experiments are shown (1 dot = 1 larva). (C-D) GFP+ area measurements in control and Mtz-treated IEC- and IEC + Μϕ co-ablated reporter larvae during (C) ablation and (D) recovery. Pooled data from 3 independent experiments are shown (1 dot = 1 larva). (E) Representative images of intestines from the IEC- and co-ablation groups during recovery (5dpf). Scale bar = 200 µm. (F) 5-bromo-2′-deoxyurinide (BrdU) incorporation in control and Mtz-treated IEC-ablated and IEC + Μϕ co-ablated groups of larvae. Scale bar = 200 µm in whole-intestine pictures and 100 µm in zoomed area. Pooled data from 2 independent experiments (1 dot = 1 larva). Different symbol shapes denote independent experiments in B, C, D, and F. Unpaired t-tests were performed per timepoint in B, Krushall-Wallis tests with Dunn’s multiple comparisons were performed in C and D, and one-way ANOVA with Tukey’s post-tests was performed in F. *p < 0.05; **p < 0.01; ***p < 0.001.