Fig. 2

Sox11a and sox11b expression overlap with inhibitory motor neuron clusters. All staining images were after “Max Intensity” z-stack projection. (A) Left: Illustration of zebrafish developing ENS separated into foregut, midgut, and hindgut. Right: Transient expression of both sox11a and sox11b in gut at 3 dpf, and only sox11a expression at 5 dpf. (Scale bar, 100 μm.) (B) Overlapping expression of sox11a, sox11b, Phox2b, and pH3 in the ENS at 3 dpf. The white dash box indicates the zoomed region on the right. Arrows indicate cells coexpressing sox11a/b, Phox2b and proliferation marker pH3. (Scale bar, 100 μm, and 50 μm for zoomed region.) (C) Coexpression pattern between sox11a, sox11b, and inhibitory motor neuron markers, adcyap1b, vip, and nos1 at 3 dpf. The white dash box indicates the zoomed region on the right. Arrows point to cells coexpressing sox11a/b and neuronal markers: hollow arrows:sox11a+/sox11b+/adcyap1b+/nos1+/vip− cells, white arrows:sox11a+/sox11b+/adcyap1b−/nos1−/vip+ cells, orange arrows:sox11a−/sox11b+/adcyap1b+/nos1+/vip− cells, purple arrows:sox11a+/sox11b+/adcyap1b+/nos1+/vip+ cells, blue arrows:sox11a+/sox11b−/adcyap1b+/nos1+/vip+ cells. (Scale bar, 100 μm, and 50 μm for zoomed region.) (D) Coexpression pattern between sox11a and only vip at 5 dpf. The white dash box indicates the zoomed region on the right. Arrows indicate cells coexpressing sox11a and vip. (Scale bar, 100 μm, and 50 μm for zoomed region.)