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Tumor histology and gross phenotypic analysis of transgenic zebrafish cohorts expressing EGFP, wtICER, and S35-41A-ICER. Representative images of zebrafish and corresponding H&E-stained tumor sections for each cohort. From left: EGFP-expressing zebrafish exhibit melanomas localized to the skin and minimal infiltration into proximal muscle tissue. Middle: wtICER-expressing zebrafish display extensive tumor growth with pronounced infiltration into the underlying muscle, indicative of an aggressive melanoma phenotype. Right: S35-41A-ICER expressing zebrafish shows some cellularity, though presents distinctly from the other samples. Gross phenotypic analysis mirrors these histological findings, with fish expressing EGFP showing moderate tumor size, wtICER fish exhibiting large, and widespread tumors, and S35-41A-ICER fish displaying smaller lesions. While the exact type of tumor was not able to be confirmed, it is believed to be melanoma. Scale bar: 70 µm.
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