Fig. 2

gal controls whole-brain activity. (A) Representative calcium signals (elavl3:GCaMP5G) recorded across the brain of 5 dpf control (blue, top) and hsp70l:gal sibling (orange, bottom). (B) Area under the curve (AUC) calculated over 1 hr recording, normalized to control (n control = 25; n hsp70l:gal = 29). (C) Galanin transcript levels in pools of control vs. hsp70l:gal brains (n = 3). (D) Number of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right). (E) Average amplitude of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. (F) Average duration of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. (G) Representative calcium signals (elavl3:GCaMP5G) recorded across the brain of 5 dpf control (blue, top) and gal-/- larva (yellow, bottom). (H) AUC calculated over 1 hr recording, normalized to control (n control = 24; n gal-/- = 24). (I) Number of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right). (J) Average amplitude of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. (K) Average duration of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. (L) Representative calcium signals (elavl3:GCaMP6f) recorded across the brain of 5 dpf control injected (blue, top) and galr1a crispants (galr1aKO) larva (green, bottom). (M) AUC calculated over 1 hr recording, normalized to control (n control = 28; n galr1a crispants = 23). (N) Galanin transcript levels in pools of control vs. galr1aKO brains (n = 3). (O) Number of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right). (P) Average amplitude of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. (Q) Average duration of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. Significance levels: ***p<0.001, **p<0.01, *p<0.05, ns = not significant (p>0.05), Wilcoxon-Mann-Whitney test (B, E, F, H, J, K, M, O, P), negative binomial regression (D).