Fig. 4

gal promotes seizures in pentylenetetrazole (PTZ) exposed larvae. (A) Averaged calcium signals (elavl3:GCaMP6f) for seizures elicited by 20 mM PTZ recorded across the brain of 5 dpf control (blue, 76 events) and hsp70l:gal (orange, 113 events) aligned by 50% of maximum amplitude. Shaded area represents SEM. (B) Time to peak calculated from beginning of aligned seizure until maximum ΔF/F0 signal. (C) Area under the curve (AUC) calculated over seizures normalized to control (n control = 76; n hsp70l:gal = 113). (D) Number of seizures per larva. (E) Amplitude of seizures per larva. (F) Duration of seizures per larva. (G) Averaged calcium signals (elavl3:GCaMP6f) for seizures elicited by 20 mM PTZ recorded across the brain of 5 dpf control (blue, 29 events) and gal-/- (yellow, 10 events) aligned by 50% of maximum amplitude. Shaded area represents SEM. (H) Time to peak calculated from beginning of aligned seizure until maximum ΔF/F0 signal. (I) AUC calculated over seizures normalized to control (n control = 29; n gal-/- = 10). (J) Number of seizures per larva. (K) Amplitude of seizures per larva. (L) Duration of seizures per larva. (M) Averaged calcium signals (elavl3:GCaMP6f) for seizures elicited by 20 mM PTZ recorded across the brain of 5 dpf control injected (blue, seven events) and galr1a crispants (galr1aKO, green, 14 events) aligned by 50% of maximum amplitude. Shaded area represents SEM. (N) Time to peak calculated from beginning of aligned seizure until maximum ΔF/F0 signal. (O) AUC calculated over seizures normalized to control (n control = 7; n galr1a crispants = 14). (P) Number of seizures per larva. (Q) Amplitude of seizures per larva. (R) Duration of seizures per larva. Significance levels: ***p<0.001, **p<0.01, *p<0.05, ns = not significant (p>0.05), Wilcoxon-MannWhitnMann Whitney (B, C, E, F, H, I, K, L, N, O, Q, R), negative binomial regression (D, J, P).