Fig. 1

gal expression correlates with whole-brain activity. (A) Representative calcium signals (elavl3:GCaMP5G) recorded across the brain of 5 dpf control (eaat2a+/+) larva (blue, top) and eaat2a-/- mutant without seizure activity (red, bottom). (B) Area under the curve (AUC) calculated and averaged over two 5 min time windows per animal and normalized to control (n control = 25; n eaat2a-/- = 12). (C) Galanin transcript levels in pools of control vs. eaat2a-/- brains (n = 3). (D) Number of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right). (E) Average amplitude of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. (F) Average duration of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. (G) Representative calcium signals (elavl3:GCaMP6f) recorded across the brain of 5 dpf control (blue, top) and larvae 2 hr after pentylenetetrazole (PTZ) exposure (PTZ rebound) (magenta, bottom). (H) AUC calculated over 1 hr recording, normalized to control (n control = 21; n PTZ rebound = 21). (I) Galanin transcript levels in pools of control vs. PTZ rebound brains (n = 3). (G) Number of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right). (J) Average amplitude of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. (K) Average duration of Ca2+ events above 5% ΔF/F0 (left) and 10% ΔF/F0 (right) per larva. Significance levels: ***p<0.001, **p<0.01, *p<0.05, ns = not significant (p>0.05), Wilcoxon-Mann-Whitney test (B, E, H, J, K), negative binomial regression (D, I), Student’s t-test (C, F).