FIGURE

Fig. 6

ID

ZDB-FIG-250725-33

Publication

Nobilleau et al., 2025 - RFC1 regulates the expansion of neural progenitors in the developing zebrafish cerebellum

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Fig. 6

Cerebellar neural progenitors accumulate DNA damage and undergo apoptosis in the absence of RFC1.A, B Combined immunofluorescence and RNAscope in situ hybridization for DNA damage marker γH2AX (green), ptf1a (orange), and atoh1a (magenta) at 2 dpf in rfc1+/+ (A, n = 5) and rfc1−/− (B, n = 5) embryos. Dorsal views (upper panels) and lateral views (lower panels) are shown. Dotted lines outline the upper rhombic lip (URL). ot optic tectum, LRL lower rhombic lip, Va valvula cerebelli. C Confocal images (1-μm optical slice) showing colocalization of apoptotic cells (TUNEL, green) and DNA damage (γH2AX, red) at 2 dpf in rfc1+/+ (n = 5) and rfc1-/- (n = 5) embryos. Dotted lines delineate the cerebellar region. D Higher magnification of selected regions from (C) (a–d), highlighting colocalization of TUNEL and γH2AX signals (white arrows) and characteristic γH2AX apoptotic ring patterns (dotted outlines).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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