Fig 1

Schematics of the wt and mutants SnRV clones.

(A) Scaled schematics of SnRV wt clone, based on [3]. Black boxes, 5’ and 3’ LTRs. Dashed lines, introns. Gray arrows and bars, exons. Black bar, the first 14 residues of 3’ORF, overlapping the env ORF. For simplicity, only one of the two putative 3’ORF mRNAs (transcript c; [3]) is shown. Upper line, map scale (in nucleotides). (B-D) SnRV mutants. The mutations’ location is portrayed relative to the map in (A). The schemes depict the junctions between the SnRV nucleotides and cognate amino acids (uppercase and bold letters) and the nested non-viral sequences (nucleotides in non-bold lowercase letters). Red arrows mark four nucleotides in the Env^mut clone (B) and a single nucleotide in the Tins clone (C), inserted to make out-of-frame mutations. Asterisks, stop codons. Green boxes (C, D) and a grid (C) illustrate the GFP and the pac ORFs, respectively.