Figure 5

Graphics of sublethal effects induced by different concentrations of N-Phosphate− FR in the zebrafish embryo toxicity test. Specifically, delayed yolk sac absorption (a), darkening of the yolk sac (b), notochord malformation (c), and yolk sac edema (d) were observed. Statistical analysis was conducted by comparing exposed groups to the control, with significance levels denoted as follows: * (p ≤ 0.05), ** (p ≤ 0.01), and *** (p ≤ 0.001). Data are presented as mean ± standard deviation. Data visualization was performed using SigmaPlot^® software, version 14.0. (e–h) Sublethal effects observed in embryos exposed to Phosphate- FR (2.5× and 5× magnification, Stemi2000-C stereomicroscope): e—negative control; f1 and g1—yolk sac edema; f2—blood stasis; g2—delayed yolk sac absorption; h1—notochord malformation and h2- darkening of the yolk sac.