Figure 4

Graphics of sublethal effects induced by different concentrations of N-Phosphate + FR in the zebrafish embryo toxicity assay. Specifically, delayed yolk sac absorption (a), darkening of the yolk sac (b), notochord malformation (c), and blood stasis (d) were observed. Statistical analysis was conducted by comparing exposed groups to the control, with significance levels denoted as follows: ** (p ≤ 0.01), and *** (p ≤ 0.001). Data are presented as mean ± standard deviation. Data visualization was performed using SigmaPlot^® software, version 14.0. (e–g) Sublethal effects observed in embryos exposed to N-Borate FR at a concentration of 1180.8 mg/L up to 96 hpf (2.5× magnification, Stemi2000-C stereomicroscope): e1—yolk sac darkening; e2—delayed yolk sac absorption; f1 and g1—blood stasis; f2 and g2—notochord malformation.