Fig. 2
- ID
- ZDB-FIG-250612-34
- Publication
- Mehrabi et al., 2025 - Optogenetic activation of hypothalamic AgRP neurons in transgenic zebrafish larvae increased food intake
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Microfluidic Chamber and Optogenetic activation. A resin 3D printed positive mold (a) was designed to create a negative mold in liquid agarose (b) inside a petri dish. The negative mold of solidified agarose (c) acts as reusable device for food particle flow experiments. Zebrafish larva can be placed and trapped in the center of the device (d) while having mouth access to food particles suspended in water. (e) In an Olympus BX51 fluorescence microscope (f) a 10 × magnification air objective was used to photoactivate the AgRP1 neurons in the hypothalamus of the larva by delivering blue light stimulation at a wavelength of 470 nm. |