FIGURE

Fig. 1

ID

ZDB-FIG-250612-33

Publication

Mehrabi et al., 2025 - Optogenetic activation of hypothalamic AgRP neurons in transgenic zebrafish larvae increased food intake

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Fig. 1

(a) Schematic of the Tol2 transgenic construct used to generate zebrafish expressing ChR2-Kaede under the control of the AgRP1 promoter. (b) Breeding strategy to generate heterozygous F2 transgenic larvae, which were subsequently stimulated with blue light to activate AgRP1 neurons. (c) Screening of a Tg(AgRP1:ChR2-Kaede) larva under an upright epifluorescent microscope using a 20 × water-immersion objective. The approximate anatomical location of AgRP1 neurons is indicated relative to the eyes. (d) High-magnification confocal imaging of the same larva using a 40 × water-immersion objective, showing five AgRP1 neurons labeled by endogenous Kaede fluorescence.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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