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Transport of germ granules to the cleavage furrows requires Rbm24a and kinesin-1. (A, B) Movements of Buc-GFP-positive granules during cleavage. (C, D) Defective movements of Buc-GFP granules in Mrbm24a mutants. (E, F) Velocity and directionality of marked granules in siblings and Mrbm24a embryos. Data are presented as mean ± SD, n = 12. ****P < 0.0001, unpaired Student’s t test. (G, H) Movement trajectories of marked granules in rbm24a-GFP KI embryos during cleavage. (I, J) Disruption of directed movements of Rbm24a-positive granules by injection of a function-blocking antibody against kinesin-1. Scale bar, 20 μm for (A–D, G–J). (K, L) Velocity and directionality of labeled granules in uninjected and kinesin-1 antibody-injected Tg(ef1α:buc-GFP) embryos. Data are presented as mean ± SD, n = 12. **P < 0.01 and ****P < 0.0001, unpaired Student’s t test. (M, N) Distribution of Piwil1 protein and nanos3 mRNA at the cleavage furrows of uninjected and kinesin-1 antibody-injected embryos. Scale bar, 20 μm. (O) Compromised localization of germ plasm mRNAs at the cleavage furrows in kinesin-1 antibody-injected embryos. Numbers in the bottom right corner indicate the ratio of analyzed embryos with the representative expression pattern shown in each panel. Scale bar, 200 μm. Source data are available online for this figure.
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