Figure 2

Loss of maternal rbm24a results in the specific absence of PGCs.

(A) Pipeline to obtain maternal rbm24a mutant embryos. Scale bar, 1 mm. (B, C) Embryos spawned by crossing wild-type female and Mrbm24a male fish fail to undergo cleavage. Scale bar, 1 mm. (D, E) Absence of testis structure in an Mrbm24a mutant adult. Scale bar, 2 mm. (F, G) Testicular tubules from an Mrbm24a adult contain no germ cells and exhibit a vacuolar shape. Scale bar, 12.5 μm. (H) Absence of germ plasm mRNA markers in Mrbm24a embryos at 24 hpf. Scale bar, 250 μm. (I–L) Absence of germ plasm protein markers Ddx4 and Piwil1 in Mrbm24a embryos at 24 hpf. Scale bar, 500 μm. (M, N) Loss of YFP signals in PGCs of an Mrbm24a embryo at 24 hpf expressing YFP fused with the 3’-UTR of nanos3 mRNA. Green serves as a pseudocolor of YFP fluorescence. Scale bar, 500 μm. (O) Loss of PGCs in Mrbm24a embryos at the shield stage, as examined by the expression of specific markers. Numbers in the bottom right corner indicate the ratio of analyzed embryos with the representative expression pattern shown in each panel. Scale bar, 250 μm. (P) Specific loss of PGCs in Mrbm24a embryos at 6 hpf, as shown by the clustering map of scRNA-seq. Source data are available online for this figure.