FIGURE

Figure 1

ID

ZDB-FIG-250604-34

Publication

Zhang et al., 2025 - Rbm24a dictates mRNA recruitment for germ granule assembly in zebrafish

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Figure 1

Rbm24a is a protein component of the germ granule.

(A) Schematic illustrating the strategy for generating the rbm24a-GFP KI line. A GFP-containing tagging cassette was introduced into the last intron of the rbm24a locus to replace the last exon. (B) Germ plasm- and germ cell-specific distribution of maternal Rbm24a protein. Scale bars, 50 μm. (C) Colocalization of Rbm24a protein with germ plasm mRNAs ddx4 and dazl as well as with the germ plasm protein Piwil1 in the germ plasm aggregates. Scale bar, 20 μm. (D) Colocalization of Rbm24a and Ddx4 in perinuclear germ granules within PGCs at 24 hpf. Scale bars, 5 μm. (E) Live imaging of Rbm24a-GFP from 8-cell stage to sphere stage. Scale bar, 200 μm. (F) Size distribution of Rbm24a-GFP- positive germ granules in the early embryos. Dots are colored to trace individual germ granules. n = 555. (G) The numbers of germ plasm condensates are reduced in an exponential decay pattern during early development (n = 3 independent biological samples). Data are presented as mean ± SD. Source data are available online for this figure.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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