CH25H expression is associated with human foetal SARS-CoV-2-associated brain microbleeds. (A) Representative images of microbleeds found in human foetal cortex by Haematoxylin and Eosin staining. Bleeds were classified into small, medium and large sizes according to area. Scale bar size is shown in each image. (B,C) Samples were categorised according to a bleeding score, based on bleed size and total bleed density. Control samples were classified as score 0, and haemorrhagic samples were classified as scores 1 and 2. Density of small, medium and large bleeds (B), and total bleed density (C) is shown for samples with bleeding scores of 0 to 2. (D) Representative images of CH25H staining (pink) and bleeds (yellow) from non-haemorrhagic or haemorrhagic samples, proximal and distal to bleeds. Scale bars: 30 µm. (E) CH25H+ cell density was quantified in samples with bleeding scores of 0 to 2. Data are mean±s.e.m. ns, non-significant; **P<0.01; ***P<0.001; determined by two-way ANOVA with Tukey's post hoc analysis multiple comparisons between scores (B), or one-way ANOVA with Kruskal–Wallis post hoc test compared to score 0 (C,E).
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