FIGURE

Fig. 5

ID

ZDB-FIG-250521-5

Publication

Windster et al., 2024 - Human enteric glia diversity in health and disease: new avenues for the treatment of Hirschsprung disease

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Fig. 5

Schwann precursor–like cells are preserved in ret-/- zebrafish and are amenable to prucalopride-induced neurogenesis. (A) Graphical representation of gut isolation for scRNA-seq of 5-dpf tg(phox2bb:GFP) wild-type (WT) and ret-/- zebrafish. (B) UMAP visualization of WT and ret–/– zebrafish ENS subsets. (C) Live imaging of 5-dpf tg(phox2bb:kaede) photoconverted ret+/– zebrafish, treated with prucalopride. New phox2bb:kaede+ cells are indicated by asterisks (∗). (D) Quantification of new phox2bb:kaede+ cells in the proximal and (E) distal regions of DMSO (n = 8) and prucalopride (n = 9) treated 5-dpf photoconverted ret+/– tg(phox2bb:kaede) zebrafish. Statistical significance was calculated using Student t tests, ns > .05; ∗∗P < .01. (F) FISH combined with immunohistochemistry shows the presence of S100B+/RELN+/HTR4+ enteric glia located within hypertrophic nerve trunks in aganglionic colon (n = 3). In the insets, S100B staining is omitted for better appreciation of the FISH signal. IPAN, Intrinsic primary afferent neuron.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ret^hu2846/+; em2Tg ret^{hu2846/hu2846}; w37Tg
Conditions:	ultraviolet light chemical treatment by environment: prucalopride, ultraviolet light
Observed In:	enteric neuron intestine distal region
Stage:	Day 5

Phenotype Detail

Acknowledgments

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