Fig. 7

(A) Effect of food deprivation on novel PEA response. Change in percentage of time spent in Q1 from (before PEA compared to during PEA exposure) of starved (N = 37 tanks of 5 fish) vs non-starved zebrafish (N = 37 tanks of 5 fish) in response to novel PEA exposure. Starved (48 h) fish spent significantly more time in Q1 near the feeding area than non-starved fish (fed immediately before experiment; t-test, t(68) = 2.329, p = 0.011 (B) Response to water puff post-training. Change in percentage of time spent in Q1 (before water puff compared to during water puff) for experimental (N = 23 tanks of 5 fish) and control (N = 23 tanks of 5 fish) groups. The water puff trial showed no significant response by either the control or experimental fish (t-test, t(22) = -0.162, p = 0.467. (C) Memory retention seven days post-training. Change in percentage of time spent in Q1 from (before PEA compared to during PEA exposure) for retention trial at 7 days post training. There was no significant difference between time spent in Q1 between the experimental (N = 12 tanks of 5 fish). and control groups (N = 12 tanks of 5 fish; t-test, t(44) = 0.979, p = 0.166). For A, B & C: [Zero indicates no change in percentage of time spent in Q1, near the feeding area. Error bars = ± S.E.M.].