Fig. 5

Movement of zebrafish groups during acquisition of an olfactory appetitive paradigm, regardless of quadrant boundaries. Zero indicates no movement from before to during PEA delivery. Initially, zebrafish in both experimental and control groups showed movement towards the site of later food delivery from their initial positions when exposed to the olfactory stimulus. This response decreased until the end of Day 2. Zebrafish in the control group showed less movement towards the feeding site in response to the olfactory stimulus as training progressed. However, the experimental group showed increased movement towards the food source. (Two-way RM ANOVA F(1, 47) = 1.584, p = 0.008; post-hoc pairwise p = 0.007; Post-hoc pairwise comparisons were performed and significant findings were indicated with a ‘*’). Trials were binned in groups of six (morning and evening of training days). Data points indicate mean distance from the food source before PEA delivery minus mean distance from the food source during PEA delivery. N = 23 tanks of 5 fish for each treatment. Error bars = ± S.E.M.