FIGURE

Fig 8

ID

ZDB-FIG-250501-37

Publication

Thomas et al., 2025 - Zebrafish Polymerase Theta and human Polymerase Theta: Orthologues with homologous function

Other Figures

All Figure Page

Back to All Figure Page

Fig 8

DNA Pol

θPD is able incorporate and extend dsDNA in the presence of Ca²⁺. Denaturing gel showing primer extension dsDNA substrate with CaCl_2. Under single turnover conditions 200 nM Pol θ PD (human or zebrafish) was preincubated with 50 nM 24/33 undamaged (A) or damaged (B) DNA substrate and reacted with 125 nM nucleotides in 10 mM CaCl₂. Reactions were carried out at 37°C for 5 minutes and products visualized on a 12% denaturing gel. Percent extended was calculated using ImageQuant software by quantifying the intensity of the extended products (n+1 and higher) divided by the intensity of the total amount of DNA.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One