PUBLICATION
Zebrafish Polymerase Theta and human Polymerase Theta: Orthologues with homologous function
- Authors
- Thomas, C., Green, S., Kimball, L., Schmidtke, I.R., Rothwell, L., Griffin, M., Par, I., Schobel, S., Palacio, Y., Towle-Weicksel, J.B., Weicksel, S.E.
- ID
- ZDB-PUB-250430-2
- Date
- 2025
- Source
- PLoS One 20: e0321886e0321886 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
- none
- PubMed
- 40299938 Full text @ PLoS One
Citation
Thomas, C., Green, S., Kimball, L., Schmidtke, I.R., Rothwell, L., Griffin, M., Par, I., Schobel, S., Palacio, Y., Towle-Weicksel, J.B., Weicksel, S.E. (2025) Zebrafish Polymerase Theta and human Polymerase Theta: Orthologues with homologous function. PLoS One. 20:e0321886e0321886.
Abstract
DNA Polymerase Theta (Pol θ) is a conserved an A-family polymerase that plays an essential role in repairing double strand breaks, through micro-homology end joining, and bypassing DNA lesions, through translesion synthesis, to protect genome integrity. Despite its essential role in DNA repair, Pol θ is inherently error-prone. Recently, key loop regions were identified to play an important role in key functions of Pol θ. Here we present a comparative structure-function study of the polymerase domain of zebrafish and human Pol θ. We show that these two proteins share a large amount of sequence and structural homology. Using a classical biochemical approach we observe that zebrafish Pol θ displays behavior characteristic of human Pol θ, including DNA template extension in the presence of different divalent metals, microhomology-mediated end joining, and translesion synthesis. These results will support future studies looking to gain insight into Pol θ function on the basis of evolutionarily conserved features.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping