FIGURE

Fig. 1

ID

ZDB-FIG-250421-121

Publication

Xiao et al., 2025 - Deficiency of psmb6 suppresses proteasome activity and promotes apoptosis in larval zebrafish brain

Other Figures

All Figure Page

Back to All Figure Page

Fig. 1

psmb6 mRNA expression pattern and generation of psmb6 mutants. (A) ISH for psmb6 at 24 hpf, 36 hpf, 48 hpf, and 3 dpf. (B) Knockout of psmb6 by CRISPR-Cas9 resulted in two different types of psmb6 mutants, with deletions of 4 bp and 42 bp (five bases are located in exon 3, and thirty-seven bases were located in intron 3–4). Sanger sequencing confirmed the 4-nt and 42-nt deletion mutations. Detection of mutant knockout efficiency was performed by Western blotting. (C) psmb6 mutants displaying cerebral hemorrhage (red arrow) and cerebral necrosis (blue arrow). Scale bars: 100 μm. (D) Phenotypic rates of two types of psmb6 mutants at 4.5 dpf. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Biochem. Biophys. Res. Commun.