FIGURE

Figure 4

ID

ZDB-FIG-250419-32

Publication

Ciapaite et al., 2025 - Broad Vitamin B₆-Related Metabolic Disturbances in a Zebrafish Model of Hypophosphatasia (TNSALP-Deficiency)

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Figure 4

Normalization of biochemical and locomotion abnormalities in 5 dpf old alpl^-/- zebrafish embryos after 72 h continuous treatment with 100 µM pyridoxine (PN). Concentrations of (a) pyridoxine (PN), (b) PLP, (c) pyridoxal (PL), and (d) 4-pyridoxic acid (4-PA) measured in whole 5 dpf old embryo extracts. Data are means from n = 4–10 pools (3 embryos per pool) per genotype ± SD. Zebrabox analysis of (e) burst activity count, (f) duration of burst activity, and (g) distance swum during burst activity in 5 dpf old embryos measured for 1 h at 28 °C in the dark. Data are means from n = 23–25 embryos per genotype and treatment ± SD. (h) γ-Aminobutyric acid (GABA) concentrations. Data are means from n = 7–15 pools (3 embryos per pool) per genotype and treatment ± SD. (i) Fold-change analysis of amino acid concentrations in untreated alpl^-/- and 100 µM PN-treated alpl^-/- zebrafish embryos compared to the corresponding WT group. Data are from n = 4 pools (3 embryos per pool). **** p < 0.0001, *** p < 0.001, ** p < 0.01, * p < 0.05, and ns—not significant (p > 0.05).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	alpl^{zf4270/zf4270} (TL)
Condition:	chemical treatment by environment: pyridoxine
Observed In:	locomotory behavior vitamin B6 metabolic process whole organism asparagine whole organism gamma-aminobutyric acid whole organism glutamine whole organism methionine
Stage:	Day 5

Phenotype Detail

Acknowledgments

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