FIGURE

Figure 6

ID

ZDB-FIG-250417-113

Publication

Oyarbide et al., 2025 - Reduced EIF6 dosage attenuates TP53 activation in models of Shwachman-Diamond syndrome

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Figure 6

Correlation of Eif6 levels with tp53 activation in sbds-KO zebrafish.

(A) Scheme of sbds^+/– crossings in the WT and Eif6 missense mutants and percentage of genotypes observed at 20 dpf. (B) Schemes of the 3 different crosses to obtain sbds WT and KO zebrafish on the eif6^+/+, eif6^+/ms, and eif6^ms/ms backgrounds. (C) Immunoblot showing Eif6 accumulation in sbds-KO zebrafish. (D) Neutrophil counts at 10 dpf. *P < 0.05, **P < 0.01, ***P < 0.001, by 1-way ANOVA with Dunnett’s test. (E) Protein levels in sbds^+/+, sbds^+/–, and sbds^–/–, in the eif6^+/+ and eif6ms/ms mutant backgrounds, along with protein quantification. (F) RT-qPCR results for sbds and eif6 (G) and the tp53 pathway. *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001, by 1-way ANOVA with Tukey’s test. Data represent the mean ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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