Figure 2

Eif6 is essential for zebrafish embryonic development.

(A) Amino acid alignment of WT and eif6 KO zebrafish; the insertion of 1 bp causes early truncation. (B) mRNA eif6 levels by RT-qPCR on the tp53^+/+ and tp53^M214K/M214K backgrounds at 5 dpf. (C) Western blots showing the absence of Eif6 protein in the eif6^–/– zebrafish larvae at 5 dpf. Note the dose effect in the eif6^+/– fish compared with their WT siblings. (D) Western blot quantification. (E) eif6 KO showed a significantly lower number of neutrophils than the WT siblings at 5 dpf. (F) Sudan black staining for neutrophils counts (original magnification, ×2.5 and ×8). (G) Survival percentages for eif6^+/+, eif6^+/–, and eif6^–/– siblings at 5, 8, and 10 dpf. *P < 0.05, **P < 0.01, and ***P < 0.001, by ANOVA. Data represent the mean ± SEM.