Fig. 6

In vivo metastasis inhibition of the rotational ZFO micromotors to A549 cells in a zebrafish xenograft model. A) The schematic diagram of the zebrafish xenograft model. B–D) Representative images of zebrafish embryos, 3 days after microinjection of Dil stained A549 upon different treatments, without ZFO, with ZFO but without rotational magnetic field, and after the action of magnetic ZFO micromotors for 90 min. (n = 5 for each experimental group) Scale bar: 200 µm. E,F)The disseminated foci number and integrated fluorescence density of disseminated foci (fluorescence density outside the yolk sac) were quantified from the 3D fluorescent images of zebrafish, the disseminated foci were marked with white triangles. All values are expressed as the mean ± SD in at least three independent experiments. ****p < 0.0001 versus control.