Figure 7

Bulk RNA sequencing of 7 dpf mtmr5-knockout versus wild-type brain-enriched samples. (A) Volcano plot displaying the differentially expressed genes in 7 days-post-fertilization (dpf) mtmr5-knockout (KO) zebrafish heads compared to wild-type (WT) siblings. A significance threshold of adjusted P-value (padj) < 0.01 and absolute log₂ fold-change >1 was used. Upregulated genes are in the top right segment and downregulated genes are in top left segment. Number of significant genes in each category are indicated (upregulated, n = 1693; downregulated, n = 347). Each dot represents one zebrafish gene. See Supplementary Table 2 for the full list of genes, padj values, and log₂fold-changes. (B) Gene ontology (GO) terms significantly enriched in the up- and down-regulated genes (padj < 0.01), sorted by the number of genes associated with the respective GO term (gene count). All enriched GO terms (padj < 0.05) are included in Supplementary Table 3. (C) Normalized read counts for the three CMT4B-associated MTMR genes: mtmr5, mtmr2 and mtmr13. ****padj < 0.00001, ns > 0.05. (D) Log₂ fold-change values for all mtmr genes with significant values indicated (padj < 0.01). mtmr5 and mtmr13 gene names are used in place of sbf1 and sbf2, respectively.