Fig. 5

Blocking Notch signaling increased neural precursor numbers in developing mouse and chicken dorsal telencephalon but not in the larval zebrafish forebrain. a Schematic diagram of experimental design of MK-0752 treatment in developing mouse cerebral cortex, immunostaining and quantitative analysis of Tbr2-positive cells (nuclei) in VZ. VZ, ventricular zone; IZ, intermediate zone; CP, cortical plate. Solid lines indicate ventricular surface. Nuclei: DAPI staining. b Schematic diagram of experimental design of MK-0752 treatment in developing chicken pallium, immunostaining, and quantitative analysis of Tuj1-positive cells (nuclei) in NZ. Tuj1-positive cells are defined as nuclei surrounded by Tuj1-positive signals (arrowhead). Dashed lines indicate the pia surface and solid lines indicate the ventricular surface. Nuclei: DAPI staining. c Schematic diagram of experimental design of MK-0752 treatment in developing larval zebrafish forebrain, in situ hybridization, and quantitative analysis of area with HuC/elavl3 signals. Close arrowheads indicate positive signals and open arrowheads indicate negative signals. Scale bars are indicated. Error bars represent standard deviation, and data points are shown in dots. *Significance with p value < 0.01 by Student’s t-test