The expression pattern of chst6 in zebrafish embryos. Whole mount in situ hybridization revealed expression of chst6 in the body of (A) 24 hpf, (B) 30 hpf, (C) 60 hpf, and (D) 72 hpf zebrafish. (E) Close-up image of the 72 hpf larval head shows strong expression in the brain and eye (arrow). The experiment was repeated three times and n = 15 for each stage. (F) Section of larvae post-in situ hybridization shows chst6 expression in the cornea (arrow); (G) larvae stained with negative control sense probe did not show any signal in the eye. (H) Representative images of 72 hpf larvae stained with chst6 sense probe (negative control). Scale bars: 100 μm.
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