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Fig. 1

ID
ZDB-FIG-250212-12
Publication
Pujol et al., 2024 - Short-term polystyrene nanoplastic exposure alters zebrafish male and female germline and reproductive outcomes, unveiling pollutant-impacted molecular pathways
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Fig. 1

PS-NPs affect spermatogenesis and sperm motility. (A) Schematic representation of spermatogenesis in zebrafish. Note how the spermatogenesis cycle is organized into cysts. (B) Immunofluorescence images representing testis cysts using antibodies against RNApol II (red) and SYCP3 (green). DNA is counter stained with DAPI (blue). Scale bar 50 µm. According to staining patterns, different cell types can be distinguished: primary spermatocytes (SpcI), round spermatids (RS) and sperm. (C) Histological sections of control (upper panel) and PS-NPs exposed (lower panel) from adult zebrafish individuals stained with PAS-Hematoxylin. Scale bar 20 µm. (D) Boxplot representing the sperm median intensity (as value per pixels) ± SD for each measured cluster in both conditions (two-way ANOVA, ***p < 0.001). A total of 1292 control sperm clusters and 1259 plastic sperm clusters were included in the analysis. Eight animals were analyzed (N = 8), representing four replicates. (E) Microscopical images of spermatic water activated samples from control individuals (upper panel) and PS-NPs exposed (lower panel). Each white dot corresponds to a sperm cell whereas each green line shows its trajectory in a specific time. No-motile sperm cells are marked with a yellow sign. Scale bar 50 µm. (F) Boxplot showing the CASA calculated sperm motility percentage median ± SD for each condition (two-way ANOVA, ***p < 0.001). Six animals were analyzed, representing two replicates.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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Reprinted from Journal of hazardous materials, 481, Pujol, G., Marín-Gual, L., González-Rodelas, L., Álvarez-González, L., Chauvigné, F., Cerdà, J., Teles, M., Roher, N., Ruiz-Herrera, A., Short-term polystyrene nanoplastic exposure alters zebrafish male and female germline and reproductive outcomes, unveiling pollutant-impacted molecular pathways, 136529136529, Copyright (2024) with permission from Elsevier. Full text @ J. Hazard. Mater.