FIGURE

Fig 5

ID

ZDB-FIG-250127-117

Publication

Ahmad et al., 2025 - The piRNA protein Asz1 is essential for germ cell and gonad development in zebrafish and exhibits differential necessities in distinct types of germ granules

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Fig 5

Asz1 is essential for piRNA granule organization ad repression of transposon expression.

A. 3 wpf asz1^+/+;Tg(ddx4:GFP) and asz1^-/-;Tg(ddx4:GFP) gonads are labeled for Ddx4 (green), Ziwi (red), ad DAPI (blue). Images are: top - merge images, middle - Ziwi channel, bottom - zoom-in images of white dashed boxes. Normal Ziwi localization to perinuclear granules is shown in asz1^+/+;Tg(ddx4:GFP) gonads. asz1^-/-;Tg(ddx4:GFP) gonads exhibit mostly undetected Ziwi granules (left), or over-aggregated and/or cytoplasmic Ziwi localization (right). Scale bars are 15 μm. B. Frequencies of Ziwi phenotypes from A are plotted. n = number of germ cells. Bars are mean ± SD. C. RT-qPCR analysis of the expression of germline transposons in 5 wpf gonads from each genotype (n = 50 gonads per genotype), as normalized for RNA loading by β-act as well as for germ cells by vasa expression (see methods). Bars are mean ± SD from biological duplicates, each made with technical replicates. Statistical analyses were tested by ANOVA.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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