Fig. 8
- ID
- ZDB-FIG-250107-25
- Publication
- Peng et al., 2024 - Identification, biological evaluation, and crystallographic analysis of coumestrol as a novel dual-specificity tyrosine-phosphorylation-regulated kinase 1A inhibitor
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Coumestrol mitigates neuronal damage caused by hyperphosphorylated tau in zebrafish larvae. (A) Representative images show motor neurite outgrowth in HuC:Kaede transgenic zebrafish larvae at 48 hpf in each group. Control group was microinjected with 50 pg of tau mRNA at 1-cell stage, and the other three experimental groups were microinjected with 50 pg of DYRK1A and 50 pg of tau mRNA. Between 20 and 48 hpf, experimental groups were immersed in 60 ?M of coumestrol, 0.08 ?M harmine or E3 medium as a vehicle control. Scale bar: 40 ?m. (B) Quantification of motor neurite outgrowth lengths (n = 18 per group) shows significantly reduced neurite outgrowth in the vehicle control group, but both coumestrol and harmine effectively protected the neurite outgrowth even with co-overexpression of DYRK1A and tau. (C) The successful rate of touch-evoked escape response in zebrafish larvae at 48 hpf (n = 30 per group) shows co-overexpression of DYRK1A and tau significantly compromised the escape reflex, but both coumestrol and harmine effectively rescued this phenotype. Data are presented as mean ± SEM. Statistical significance was determined by Kruskal-Wallis test followed by Dunn's post-hoc test (* p < 0.05, ** p < 0.01, *** p < 0.005). |
Reprinted from International journal of biological macromolecules, 282(Pt 4), Peng, C.H., Hwang, T.L., Hung, S.C., Tu, H.J., Tseng, Y.T., Lin, T.E., Lee, C.C., Tseng, Y.C., Ko, C.Y., Yen, S.C., Hsu, K.C., Pan, S.L., HuangFu, W.C., Identification, biological evaluation, and crystallographic analysis of coumestrol as a novel dual-specificity tyrosine-phosphorylation-regulated kinase 1A inhibitor, 136860, Copyright (2024) with permission from Elsevier. Full text @ Int. J. Biol. Macromol.