FIGURE

Fig. 5

ID

ZDB-FIG-250103-9

Publication

Niu et al., 2024 - An unexpected role of IL10 in mesoderm induction and differentiation from pluripotent stem cells: Implications in zebrafish angiogenic sprouting, vascular organoid development, and therapeutic angiogenesis

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Fig. 5

IL10 knockdown caused defective mesoderm formation, angiogenic sprouting, and vascular development. Tg(Flk1:EGFP) zebrafish embryos were injected with the indicated amounts (3, 4, or 5 ng) of standard control morpholino (std-MO) or IL10 target morpholino (IL10-MO) and were inspected at 32 and 48 hours post fertilization (HPF), respectively. (A-B) Representative fluorescent images showing different types of vascular defects in IL10-MO-injected Tg(Flk1:EGFP) embryos at 32 (A) and 48 (B) HPF, including normal, moderate (short and incomplete ISVs, red arrowheads), and severe (lack or inadequate ISV sprouting, red arrows). (C-D) Percentage of defective ISVs. Numbers on the top of bars indicate the total numbers of embryos with indicated treatments. (E) Total numbers of ISVs in Tg(Flk1:EGFP) zebrafish embryos with indicated treatments. ISV, intersegmental vessel; DA, dorsal aorta; DLAV, dorsal longitudinal anastomotic vessel; PCV, posterior cardinal vein.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Eur. J. Cell Biol.